anti ob r Search Results


anti human leptin receptor antibody  (Miltenyi Biotec)
92
Miltenyi Biotec anti human leptin receptor antibody
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Anti Human Leptin Receptor Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/pmc09911755-224-2-9?v=Miltenyi+Biotec
Average 92 stars, based on 1 article reviews
anti human leptin receptor antibody - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
leptin receptor  (Alomone Labs)
92
Alomone Labs leptin receptor
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Leptin Receptor, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/pm39536931-61-157-159?v=Alomone+Labs
Average 92 stars, based on 1 article reviews
leptin receptor - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
ob r  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ob r
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Ob R, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/ppr0600146-65-6-33?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
ob r - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
lepr  (Proteintech)
94
Proteintech lepr
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Lepr, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/10__1016_slash_j__placenta__2026__01__013-111-29-33?v=Proteintech
Average 94 stars, based on 1 article reviews
lepr - by Bioz Stars, 2026-07
94/100 stars
  Buy from Supplier
rabbit anti leptin receptor  (Boster Bio)
93
Boster Bio rabbit anti leptin receptor
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Rabbit Anti Leptin Receptor, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/pm27600729-43-17-23?v=Boster+Bio
Average 93 stars, based on 1 article reviews
rabbit anti leptin receptor - by Bioz Stars, 2026-07
93/100 stars
  Buy from Supplier
obr-e1 polyclonal rabbit anti-mouse antibody  (Torrey Pines Biolabs)
90
Torrey Pines Biolabs obr-e1 polyclonal rabbit anti-mouse antibody
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Obr E1 Polyclonal Rabbit Anti Mouse Antibody, supplied by Torrey Pines Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/pm11349006-78-49-54?v=Torrey+Pines+Biolabs
Average 90 stars, based on 1 article reviews
obr-e1 polyclonal rabbit anti-mouse antibody - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
anti-leptin receptor-antibody ob-r  (ZellBio GmbH)
90
ZellBio GmbH anti-leptin receptor-antibody ob-r
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Anti Leptin Receptor Antibody Ob R, supplied by ZellBio GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/pmc10628377-85-6-9?v=ZellBio+GmbH
Average 90 stars, based on 1 article reviews
anti-leptin receptor-antibody ob-r - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
leptin receptor ob r  (St Johns Laboratory)
90
St Johns Laboratory leptin receptor ob r
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Leptin Receptor Ob R, supplied by St Johns Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/pm34763177-65-0-7?v=St+Johns+Laboratory
Average 90 stars, based on 1 article reviews
leptin receptor ob r - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
rabbit polyclonal leptin receptor antibody  (Boster Bio)
92
Boster Bio rabbit polyclonal leptin receptor antibody
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Rabbit Polyclonal Leptin Receptor Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/pm37769981-81-26-35?v=Boster+Bio
Average 92 stars, based on 1 article reviews
rabbit polyclonal leptin receptor antibody - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
monoclonal antibody  (Boster Bio)
90
Boster Bio monoclonal antibody
A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without <t>anti-leptin</t> antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of <t>leptin</t> <t>receptor</t> was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.
Monoclonal Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ob+r/pm28868943-44-21-23?v=Boster+Bio
Average 90 stars, based on 1 article reviews
monoclonal antibody - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
anti-ob-r (l662) lepr antibody  (Boster Bio)
N/A
Boster Bio Anti-Ob-R (L662) LEPR Antibody catalog # A00350-2. Tested in WB,IHC applications. This antibody reacts with Human.
   Buy from Supplier
anti-ob-r lepr antibody  (Boster Bio)
N/A
Boster Bio Anti-Ob-R LEPR Antibody catalog # A00350-3. Tested in WB,ICC/IF applications. This antibody reacts with Human,Rat.
   Buy from Supplier

Image Search Results


A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without anti-leptin antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of leptin receptor was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.

Journal: Cell Death & Disease

Article Title: Elevated fatty acid β-oxidation by leptin contributes to the proinflammatory characteristics of fibroblast-like synoviocytes from RA patients via LKB1-AMPK pathway

doi: 10.1038/s41419-023-05641-2

Figure Lengend Snippet: A RA-FLS were stimulated with vehicle, serum from RA patient and HC with or without anti-leptin antibody (500 ng/mL), together with serum from RA patient and isotype antibody (500 ng/mL). Cells were harvested at 24 h and mRNA expression of the indicated genes was determined with real-time PCR analysis ( n = 5). B Measurement of CPT-1A, CPT-1B and CPT-1C mRNA expressions in RA-FLS by real-time PCR (left, n = 5). RA-FLS were treated with vehicle, serum from HC with or without leptin, serum from rheumatoid arthritis with or without leptin antibody, isotype antibody, and levels of CPT-1A were determined by western blot ( n = 4). C Expression of leptin receptor was assessed in RA-FLS treated by vehicle, serum from HC and RA patient with real-time PCR analysis ( n = 5), western blot and immunofluorescence assay (scale bar = 200 μm, n = 4). D CPT-1A was tested with real-time PCR in RA-FLS stimulated by leptin (100 ng/mL), anti-leptin antibody (500 ng/mL) and isotype antibody (500 ng/mL) for 24 h, respectively ( n = 5). CPT-1A protein expression was also detected at 48 h after indicated stimulations ( n = 4). Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, ns means no significance compared with control.

Article Snippet: Incubate with anti-human leptin receptor antibody (cat. no. 130-125-241, Miltenyi Biotec, Germany, 1:100 diluted) at room temperature for 30 min.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence, Control

A Protein–protein interactions (PPI) among leptin, leptin receptor and major metabolic pathways was consturcted. Only the PPI with the confidence of >0.4 was kept. B AMPK total and phosphorylated protein at T172 (pAMPK), LKB1 and CAMKK protein levels were compared in RA-FLS treated in the presence or absence of leptin by western blot ( n = 4). C Assessment of LKB1 protein level in RA-FLS processed with vehicle, anti-leptin antibody and isotype antibody by western blot ( n = 4). D AMPK total and pAMPK in NC or down-regulation of LKB1 with siRNA in the presence or absence of leptin was detected at 48 h ( n = 4). E CPT-1A gene ( n = 5) and protein ( n = 4) levels in RA-FLS stimulated by leptin with or without compound C (CC, 5 μM) was evaluated. Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, *** P < 0.001, **** P < 0.0001, ns means no significance compared with control.

Journal: Cell Death & Disease

Article Title: Elevated fatty acid β-oxidation by leptin contributes to the proinflammatory characteristics of fibroblast-like synoviocytes from RA patients via LKB1-AMPK pathway

doi: 10.1038/s41419-023-05641-2

Figure Lengend Snippet: A Protein–protein interactions (PPI) among leptin, leptin receptor and major metabolic pathways was consturcted. Only the PPI with the confidence of >0.4 was kept. B AMPK total and phosphorylated protein at T172 (pAMPK), LKB1 and CAMKK protein levels were compared in RA-FLS treated in the presence or absence of leptin by western blot ( n = 4). C Assessment of LKB1 protein level in RA-FLS processed with vehicle, anti-leptin antibody and isotype antibody by western blot ( n = 4). D AMPK total and pAMPK in NC or down-regulation of LKB1 with siRNA in the presence or absence of leptin was detected at 48 h ( n = 4). E CPT-1A gene ( n = 5) and protein ( n = 4) levels in RA-FLS stimulated by leptin with or without compound C (CC, 5 μM) was evaluated. Data are means ± SEM of three independent experiments, statistical significance was determined as ** P < 0.01, * P < 0.05, *** P < 0.001, **** P < 0.0001, ns means no significance compared with control.

Article Snippet: Incubate with anti-human leptin receptor antibody (cat. no. 130-125-241, Miltenyi Biotec, Germany, 1:100 diluted) at room temperature for 30 min.

Techniques: Protein-Protein interactions, Western Blot, Control